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R. & Rubinsztein-Dunlop, H. (2000). Effects associated with bubble formation in optical trapping, J. Mod. Opt. 47: 1575–1585. 1080/09500340008235124 Boyd, D. , Adleman, J. , Goodwin, D. G. & Psaltis, D. (2008). Chemical separations by bubble-assisted interphase mass-transfer, Anal. Chem. 80: 2452–2456. 1021/ac702174t Boyd, R. D. & Vest, C. M. (1975). Onset of convection due to horizontal laser beams, Appl. Phys. Lett. 26: 287–288. APL/26/287/1 Bratukhin, Y. K. & Zuev, A. L. (1984). Thermocapillary drift of an air bubble in a horizontal Hele-Shaw cell, Fluid Dyn.

Reactions in droplets in microfluidic channels, Angew. Chem. Int. Ed. 45: 7336–7356. 200601554 Microfluidic Transport DrivenEffects by Opto-Thermal Effects Microfluidic Transport Driven by Opto-Thermal 27 25 Squires, T. M. & Quake, S. R. (2005). Microfluidics: fluid physics at the nanoliter scale, Rev. Mod. Phys. 77: 977–1026. 977 Stevenson, D. , Gunn-Moore, F. , Campbell, P. & Dholakia, K. (2010). Single cell optical transfection, J. R. Soc. Interface 7: 863–871. -H. & Takeuchi, S. (2007). A trap-and-release integrated microfluidic system for dynamic microarray applications, Proc.

21. Comparison of SeS-PIV image (upper) and micro-PIV raw images (bottom) One may find better contrast of the image with focused seeding. This is caused by the lack of background, as no tracers are present outside focused, controlled tracer streams of known geometry. Moreover, the tracers are flowing in a thin layer, thinner than the depth of focus, so their images lack the diffraction rings as opposed to out-of-focus particles present in the micro-PIV picture. The visible blurred area near the obstacle in SeS-PIV picture is due to the three-dimensional deformation of focused stream.

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