By Hidenori Akiyama, Richard Heller

This e-book specializes in bioelectrics, a brand new multidisciplinary box encompassing engineering and biology with purposes to the clinical, environmental, meals, strength, and biotechnological fields. at this time, 15 universities and institutes in Japan, the united states and the european contain the foreign Consortium of Bioelectrics, meant to enhance this novel and critical learn box. This booklet will function an introductory source for younger scientists and likewise as a textbook to be used through either undergraduate and graduate scholars – the world’s first such paintings exclusively dedicated to bioelectrics.

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IEEE Trans. Dielect. Elect. Insulation. 16, 1224–1235 (2009a) 98. : Cancellation of cellular responses to nanoelectroporation by reversing the stimulus polarity. Cell. Mol. Life Sci. 71(22), 4431–4441 (2014) 99. : Ion transport into cells exposed to monopolar and bipolar nanosecond pulses. Bioelectrochemistry 103, 44–51 (2015) 100. : The effect of pulsed electric fields on biological cells: experiments and applications. Trans. Plasma Sci. 25, 284–292 (1997) 101. : Bacterial decontamination of liquids with pulsed electric fields.

Some DNA repair was observed when permeabilization of cells for the comet assay was delayed for an hour. At this time it is not known if DNA damage is a primary (direct) effect of intense nanosecond electric fields or a secondary effect caused by membrane permeabilization and/or mediated by apoptosis-inducing factor (AIF) released from mitochondria or following caspase cleavage of DNA. A more detailed discussion on cell death mechanisms is given in Sect. 10 by S. Beebe. Since earth life forms did not evolve in the presence of nsPEFs, it was of interest to determine what cellular systems would function in response to nsPEFs.

3, 263–282 (2012) 70. : Molecular monitoring of inactivation efficiencies of bacteria during pulsed electric field treatment of clinical wastewater. J. Appl. Microbiol. 105(6), 2035–2045 (2008) 71. : High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16(13), 6127–6145 (1988) 72. : High-voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA. Proc. Natl. Acad. Sci. U. S. A. 85(3), 856–860 (1988) 73. : High efficiency transformation of intact yeast cells by electric field pulses.

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