By M. T. Silva (auth.), Robert M. Burton, Francisco Carvalho Guerra (eds.)
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Extra resources for Biomembranes: Dynamics and Biology
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30:192 (1966). F. Op den Kamp Department of Biochemistry University of Utrecht Padualaan 8 Utrecht, The Netherlands INTRODUCTION It is only about one decade ago when it was for the first time proposed that the phospholipids in a biological membrane might be distributed over both halves of the bilayer in a highly asymmetric fashion. This proposal of Bretscher was based on the observations that treatment of intact erythrocytes with NH2-grouP specific reagents does not result in the labeling of appreciable quantities of the amino-phospholipids, phosphatidylethanolamine (PE) and phosphatidylserine (PS), whereas these phospholipids could be easily labeled when open ghost membranes were exposed to the reagents (1).