By Teresa V. Bowman, Akil A. Merchant (auth.), Meral Beksac (eds.)

Molecular equipment: Stem phone Transplantation offers a compendium of state of the art examine at the molecular steps excited by hematopoietic stem phone (HSC) activation and self-renewal. The emergence of HLA typing, and the optimistic effect it has had at the good fortune of scientific transplantation is emphasised and mentioned by means of striking stem mobile researchers.

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16. Add the concentrated lentiviral supernatant, to the dilution determined in step 13 above. Remove the cells to a tissue culture dish with adequate surface area to allow the cells to lie in single cell thickness at the bottom. Incubate at 37°C/5% CO2 for 24 h. DAY 6: 17. If there is a fluorescent protein, assess transduction efficiency using flow cytometry. 18. If transduction is adequate, proceed to transplantation as described later. Cell doses of 10,000–50,000 labeled cells into SCID-Hu mice have been used.

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9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 33 means of pinhole SPECT in a TNBS colitis mouse model. Nucl. Med. Biol. 31, 93–101. Hildebrandt, I. J. and Gambhir, S. S. (2004) Molecular imaging applications for immunology. Clin. Immunol. 111, 210–224. , et al. (2004) Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord. J. Neurosci. Res. 76, 232–243. , et al. (2002) Tracking transplanted stem cell migration using bifunctional, contrast agent-enhanced, magnetic resonance imaging.

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